Journal: PLoS ONE

Article Title: The Study on Newly Developed McAb NJ001 Specific to Non-Small Cell Lung Cancer and Its Biological Characteristics

doi: 10.1371/journal.pone.0033009

Figure Lengend Snippet: The cell suspensions (2×10 4 cells) mixed with 0.3% agarose and different concentrations of NJ001 or MCA2849 were layered on the top of culture media in 6-well culture plates and allowed to grow for 2 weeks before colonies were counted. Representative contrast images were shown. (A) 0 µg/mL NJ001, (B) 100 µg/mL NJ001, (C) 200 µg/mL NJ001, (D) 400 µg/mL NJ001, (E) 0 µg/mL MCA2849, (F) 100 µg/mL MCA2849, (G) 200 µg/mL MCA2849, (H) 400 µg/mL MCA2849.

Article Snippet: Anti-tetanus McAb (MCA2849) (AbD Serotec, Germany) was used as irrelevant McAb in this study.

Techniques:

Journal: PLoS ONE

Article Title: The Study on Newly Developed McAb NJ001 Specific to Non-Small Cell Lung Cancer and Its Biological Characteristics

doi: 10.1371/journal.pone.0033009

Figure Lengend Snippet: Results of colony formation of SPC-A1 cells treated by NJ001 or MCA2849 in soft agar.

Article Snippet: Anti-tetanus McAb (MCA2849) (AbD Serotec, Germany) was used as irrelevant McAb in this study.

Techniques:

Journal: PLoS ONE

Article Title: The Study on Newly Developed McAb NJ001 Specific to Non-Small Cell Lung Cancer and Its Biological Characteristics

doi: 10.1371/journal.pone.0033009

Figure Lengend Snippet: SPC-A1 cells were cultured with or without 200 µg/mL NJ001 or MCA2849 for 24 h and 48 h. (A) Morphological changes in SPC-A1 cells were observed under inverted microscope (×100). a, 24 h NJ001; b, 24 h MCA2849; c, 24 h McAb free; d, 48 h NJ001; e, 48 h MCA2849; f, 48 h McAb free. (B) Apoptosis was analyzed by flow cytometry. (C) Each column and error bar represents the mean ± SD of three independent experiments (** P <0.001). The amount of late apoptosis was determined as the percentage of Annexin V + /PI + cells.

Article Snippet: Anti-tetanus McAb (MCA2849) (AbD Serotec, Germany) was used as irrelevant McAb in this study.

Techniques: Cell Culture, Inverted Microscopy, Flow Cytometry

Journal: Frontiers in Immunology

Article Title: Silencing SOCS1 via Liposome-Packed siRNA Sustains TLR4-Ligand Adjuvant

doi: 10.3389/fimmu.2019.01279

Figure Lengend Snippet: (A) Monocytes were stimulated with L-MPLA and treated with liposome-packed Tetanus light chain toxin (L-TeTxLC). The next day lysates for western blot analyses were produced and blottet with anti-TeTxLC-specific and anti-actin- antibodies. (B) L-MPLA-activated, TeTxLC-loaded monocytes with or without L-αSOCS1-siRNA were co-cultured with CFSE-labeled CD3 + T cells from the same donor. After 5–6 days T cell proliferation was determined by flow cytometry (FITC signal). Shown are dot blots and (C) Quantifications of (B) and three more experiments. Shown is the mean + std, n = 4. Statistics: Mann– Whitney U test, one-tailed, confidence intervals 95%, * p ≤ 0.05; ns, not significant. Line above the bars depicts the compared groups. Additionally performed test: Kruskal-Wallis. Number of groups: 5; P -value 0.0729; sum value: not significant; the medians do not vary signif. ( P < 0.05). (D) CFSE labeled CD4 + or CD8 + T cells were co-cultured with L-MPLA ± L- αSOCS1 siRNA for 6 days and analyzed at a FACSCanto for fading FITC signal. Shown are the overlays of histogram produced with WEASEL flow cytometry software.

Article Snippet: Probing was performed with antibodies: Anti-p(y701) STAT3, anti-STAT3, anti-p65, anti-Actin (Cell Signaling Technology, Danvers, MA, USA), and anti-Tetanus Toxin light chain (bioTechne GmbH, Wiesbaden, Germany).

Techniques: Western Blot, Produced, Cell Culture, Labeling, Flow Cytometry, MANN-WHITNEY, One-tailed Test, Software